您当前所在的位置:首页>>兽医科技进展

禽流感病毒实时荧光定量RT-PCR检测方法的建立

点击数:20170  时间:2019-05-10 13:39:09  来源: 中国动物检疫      作者: 王楷宬,王素春,朱琳等

为满足禽流感病毒高通量快速检测的需要,建立了一种能够检测各亚型禽流感病毒的实时荧光定量RT-PCR检测方法,并对该方法的特异性和灵敏度进行评估,使用该方法与农业行业标准NY/T772—2013中的禽流感病毒RT-PCR方法同时进行临床样品检测。结果显示,该方法检测耗时短、特异性好,检测下限达10-4 ng/μL,与传统的RT-PCR方法阳性符合率为100%。结果表明,本方法能实现对禽流感病毒的安全、特异、快速、灵敏、简单、高通量检测,从而弥补了现有传统检测技术的不足。



Establishment of Real-time RT-PCR for Detection of AvianInfluenza Virus

In order to achieve the high-throughput and rapid detection ofavian influenza virus(AIV),a real-time fluorescent quantitative RT-PCR method was establishedfor detecting all subtypes of AIV. Then its specificity and sensitivity wereevaluated,and some clinical samples were testedsimultaneously by the established method and the conventional RT-PCR stipulatedin the agricultural industry standard NY/T772—2013. Results showed that thedetection limit of the established method could reach 10-4 ng/μL with shortdetection time and better specificity,and the positivecoincidence rate was 100% as compared with traditional RT-PCR method. Inconclusion,the established real-time RT-PCR method wassafe,specific,rapid,sensitive and simple,and could achieve thehigh-throughput detection for AIV,which covered thedeficiency of current traditional detection technologies.




全文下载链接:

    http://kns.cnki.net/KCMS/detail/37.1246.S.20190422.1018.032.html



网站首页 | 网站声明 | 联系我们 | 网站帮助 |

版权所有及制作维护:中国动物卫生与流行病学中心

鲁ICP备08016398号  公安机关备案号 37020302370412

网站保留所有权,未经许可不得复制、镜像