Cloning，Prokaryotic Expression and Bioinformatics Analysis of VjbR Gene of Brucella melitensis Rev.1 Strain
In order to construct the expression vector（pET-30a-VjbR）to express the VjbR protein of Brucella，and to analyze the bioinformatics information of the protein by biological software so as to lay a foundation for further relevant study. Taking the genome of Brucella melitensis Rev.1 strain as a template，VjbR gene sequence was amplified and then cloned into the prokaryotic expression vector，pET-30a（+），to obtain the recombinant plasmid，pET-30a-VjbR，then the prokaryotic expression，western-blot and bioinformatics analysis were respectively carried out. The results showed that VjbR gene was successfully cloned and expressed；the purer protein was received after SDS-PAGE analysis and purification for the expressed products；it was detected that the expressed protein could specially react with the positive sheep serum through western-blot，showing good reactivity；according to the analysis by series of bioinformatics software，it was found that VjbR protein was with no any transmembrane domain or signal peptide，it had 15 antigenic determinants. In the secondary structure，the number of α-spiral amino acids was 13，accounting for 49.81%. In conclusion，the successful expression and purification of VjbR gene of Brucella would lay a foundation for further preparation of monoclonal antibodies and development of iELISA kit.