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山东省3家鹦鹉养殖场多瘤病和喙羽病的病原检测与序列分析

8697 2020-03-12 13:14:05 吴汝娟,李玉杰,谢立香,等 中国动物检疫
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2018年山东省某养殖场发生大量幼龄鹦鹉死亡事件,疑似为病毒感染。为探寻病因,开展了该场及省内另外两个鹦鹉养殖场的流行病学调查。利用PCR技术对临床样品中提取的DNA或RNA进行检测,结果发现新城疫与禽流感病毒均呈阴性,而禽多瘤病毒(APV1)与鹦鹉喙羽病病毒(PBFDV)呈现为强阳性,由此推断此3个鹦鹉养殖场存在APV1和PBFDV感染,平均病毒检出率分别为67.9%与71.4%,共感染检出率率为58.0%。对阳性样品进化全基因分析发现:区域内的PBFDV流行毒株同源性高,与该地区早期报道的毒株亲缘关系较为接近;APV1的VP1基因同源性较高,与欧洲分离毒株亲缘关系较为接近,说明我国流行的APV1或来源于进口鹦鹉。本研究警示,需要加强鹦鹉疾病防控,严格鹦鹉进出口检疫,并制定和健全标准的鹦鹉病检疫程序。

Pathogen Detection and Sequence Analysis of Aves Polyomavirus 1 and Psittacine Beak & Feather Disease Virus in Three Parrot Farms in Shandong Province

In 2018,a large number of young parrots died in a farm in Shandong Province,which was suspected of being infected with virus. In order to find out relevant causes,an epidemiological investigation was carried out in the infected farm and other two farms in Shandong Province. DNA or RNA extracted from clinical samples was tested by PCR,it was found that Newcastle disease virus(NDV)and avian influenza virus(AIV)were detected negative,while Aves polyomavirus 1(APV1)and psittacine beak & feather disease virus(PBFDV)were detected strongly positive,therefore,a conclusion was given that parrots in the three farms had already been infected with APV1 and PBFDV,with average positive rate of 67.9% and 71.4% respectively,and the average co-infection rate was 58.0%. As per the results of whole-genome analysis on these positive samples,the prevalent strains of PBFDV in the region shared high homology,which was more similar to those reported earlier;the VP1 genes of APV1 were with higher homology,which were more closer to those isolated in Europe,indicating that the APV1 prevalent in China might be introduced by imported parrots. Hence,it was necessary to strengthen the prevention of parrot diseases and increase quarantine indicators related to imported parrots in China.

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