Pathogen Detection and Sequence Analysis of Aves Polyomavirus 1 and Psittacine Beak & Feather Disease Virus in Three Parrot Farms in Shandong Province
In 2018，a large number of young parrots died in a farm in Shandong Province，which was suspected of being infected with virus. In order to find out relevant causes，an epidemiological investigation was carried out in the infected farm and other two farms in Shandong Province. DNA or RNA extracted from clinical samples was tested by PCR，it was found that Newcastle disease virus（NDV）and avian influenza virus（AIV）were detected negative，while Aves polyomavirus 1（APV1）and psittacine beak & feather disease virus（PBFDV）were detected strongly positive，therefore，a conclusion was given that parrots in the three farms had already been infected with APV1 and PBFDV，with average positive rate of 67.9% and 71.4% respectively，and the average co-infection rate was 58.0%. As per the results of whole-genome analysis on these positive samples，the prevalent strains of PBFDV in the region shared high homology，which was more similar to those reported earlier；the VP1 genes of APV1 were with higher homology，which were more closer to those isolated in Europe，indicating that the APV1 prevalent in China might be introduced by imported parrots. Hence，it was necessary to strengthen the prevention of parrot diseases and increase quarantine indicators related to imported parrots in China.